Nanomechanics of Fluorescent DNA Dyes on DNA Investigated by Magnetic Tweezers
نویسندگان
چکیده
منابع مشابه
Design of magnetic tweezers for DNA manipulation
We study different configurations of permanent magnets and ferromagnetic circuit, in order to optimize the magnetic field for the so-called “magnetic tweezers” technique, for studing mechanical properties of DNA molecules. The magnetic field is used to pull and twist a micron-sized superparamagnetic bead, tethered to a microscope slide surface by a DNA molecule. The force applied to the bead mu...
متن کاملMagnetic Forces and DNA Mechanics in Multiplexed Magnetic Tweezers
Magnetic tweezers (MT) are a powerful tool for the study of DNA-enzyme interactions. Both the magnet-based manipulation and the camera-based detection used in MT are well suited for multiplexed measurements. Here, we systematically address challenges related to scaling of multiplexed magnetic tweezers (MMT) towards high levels of parallelization where large numbers of molecules (say 10(3)) are ...
متن کاملEarly detection of Chlamydia trachomatis using fluorescent, DNA binding dyes.
HeLa 229 cells were infected with genital tract strains of Chlamydia trachomatis. After incubation for varying times the infected cells were fixed and stained with the fluorescent DNA binding dyes Hoechst 33258 or DAPI for comparison with conventional Giemsa stain. Fluorochrome-treated preparations were examined by incident ultraviolet fluorescence microscopy and the Giemsa-stained preparations...
متن کاملEarly detection of Chlamydia trachomatis using fluorescent , DNA binding dyes
HeLa 229 cells were infected with genital tract strains of Chlamydia trachomatis. After incubation for varying times the infected cells were fixed and stained with the fluorescent DNA binding dyes Hoechst 33258 or DAPI for comparison with conventional Giemsa stain. Fluorochrome-treated preparations were examined by incident ultraviolet fluorescence microscopy and the Giemsa-stained preparations...
متن کاملDNA Tweezers Based on Semantics of DNA Strand Graph
Because of the limitations of classical silicon based computational technology, several alternatives to traditional method in form of unconventional computing have been proposed. In this paper we will focus on DNA computing which is showing the possibility of excellence for its massive parallelism, potential for information storage, speed and energy efficiency. In this paper we will describe ho...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2016
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2016.08.042